DNase I Coatings for Robust and Cytocompatible Dental Implants

This research examines the effectiveness of deoxyribonuclease I (DNase I) coatings on titanium implants to prevent infections by breaking down the structural matrix of bacterial biofilms. Researchers utilized alternating current electrophoretic deposition (AC-EPD) to apply these coatings onto titanium surfaces pre-treated with polydopamine, comparing this method to traditional dip-coating. The results demonstrate that AC-EPD creates a more mechanically stable layer that resists degradation during intense ultrasonication while maintaining its chemical integrity. Furthermore, in vitro testing with human oral keratinocytes confirmed that the coatings are not only safe but actually enhance cell adhesion and spreading. By providing both biocompatibility and a robust defense against pathogens, this technology represents a significant advancement for the longevity of dental and orthopedic implants. Overall, the study concludes that these enzymatically active surfaces offer a durable and non-toxic alternative to conventional antibiotics in clinical settings.